E-ISSN 2578-7322
 

Original Research 


Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente.

Abstract
ABSTRACT
Aim: The schistosomicidal activity of C. umbellatum leaves aqueous extract has been previously demonstrated. The present study was performed to establish the acute and sub-chronic oral toxicity profile of C. umbellatum leaves aqueous extract in mice.
Methods: For acute oral toxicity study, C. umbellatum leaves aqueous extract (CuAE) was administered per os to female mice at a single dose of 2000 mg/kg. Animals were observed for 14 days in order to identify signs of toxicity or death. In the repeated dose 28-day oral toxicity study, the extract was administered daily and per os to female and male mice at doses of 200, 400 and 800 mg/kg for 28 consecutive days. A satellite group was also set up. Body weight was measured weekly. Hematological, biochemical and histopathological parameters were analyzed.
Results: CuAE at 2000 mg/kg produced no sign of toxicity or mortality. In the sub-chronic toxicity study, no mortality, no significant change of the body weight, the relative organ weights and the hematological parameters were recorded in all treated mice. CuAE at 400 mg/kg significantly increased transaminases activities in male mice. Except for creatinine and HDL-cholesterol which concentrations increased after administration of CuAE at 800 mg/kg, the levels of others biochemical markers didn’t change. Histopathological abnormalities found in the kidneys were reversible.
Conclusion: These results indicated that the LD50 of CuAE is greater than 2000 mg/kg and CuAE, therefore, belongs to the category five of relatively non-toxic substances. From the sub-chronic toxicity study, the no-observed-adverse-effect level (NOAEL) of CuAE for both male and female mice was considered to be 200 mg/kg/day.

Key words: Clerodendrum umbellatum, acute oral toxicity, repeated dose 28-day oral toxicity, NOAEL, mice.


 
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Pubmed Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente. Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice . A J Physiol Biochem Pharmacol. 2018; 7(2): 75-85. doi:10.5455/ajpbp.20180616012821


Web Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente. Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice . http://www.ajpbp.com/?mno=302026 [Access: November 19, 2019]. doi:10.5455/ajpbp.20180616012821


AMA (American Medical Association) Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente. Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice . A J Physiol Biochem Pharmacol. 2018; 7(2): 75-85. doi:10.5455/ajpbp.20180616012821



Vancouver/ICMJE Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente. Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice . A J Physiol Biochem Pharmacol. (2018), [cited November 19, 2019]; 7(2): 75-85. doi:10.5455/ajpbp.20180616012821



Harvard Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente (2018) Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice . A J Physiol Biochem Pharmacol, 7 (2), 75-85. doi:10.5455/ajpbp.20180616012821



Turabian Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente. 2018. Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice . American Journal of Physiology, Biochemistry and Pharmacology, 7 (2), 75-85. doi:10.5455/ajpbp.20180616012821



Chicago Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente. "Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice ." American Journal of Physiology, Biochemistry and Pharmacology 7 (2018), 75-85. doi:10.5455/ajpbp.20180616012821



MLA (The Modern Language Association) Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente. "Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice ." American Journal of Physiology, Biochemistry and Pharmacology 7.2 (2018), 75-85. Print. doi:10.5455/ajpbp.20180616012821



APA (American Psychological Association) Style

Hermine Boukeng Jatsa, Joseph Bertin Kadji Fassi, Mérimé Christian Kenfack, Nestor Gipwe Feussom, Mireille Poumeni Kameni, Nadège Distele Simo, Emilienne Tienga Nkondo, Alain Bertrand Dongmo, Louis-Albert Tchuem Tchuente (2018) Acute and sub-chronic oral toxicity studies of the leaves aqueous extract of Clerodendrum umbellatum Poir. on mice . American Journal of Physiology, Biochemistry and Pharmacology, 7 (2), 75-85. doi:10.5455/ajpbp.20180616012821





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