E-ISSN 2578-7322
 

Original Research 


Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi.

Abstract
Objective: This study was designed to evaluate the effects of topically applied hexane fraction and aqueous extract of C. afer leaves balm and cream formula on xylene-induced ear edema.
Methods: In vitro anti-inflammatory study was carried out using thermally-induced protein denaturation and human erythrocyte membrane stabilization assays while in vivo anti-inflammatory study was performed using xylene-induced ear edema in rat model.
Results: Hexane fraction of C. afer leaves significantly inhibited thermally-induced protein denaturation and stabilized human erythrocyte membrane against hypotonicity-induced hemolysis when compared with aqueous extract of C. afer leaves. Furthermore, in vivo study showed that 10% and 20% w/w hexane fraction of C. afer leaves balm formula at 360 min significantly suppressed xylene-induced ear edema by 82.02% (0.16 0.01 mm) and 96.62% (0.03 0.01 mm) respectively when compared with 10% and 20% w/w aqueous extract of C. afer leaves cream formula with 55.06% (0.40 0.01 mm) and 67.42% (0.29 0.02 mm) inhibitions respectively.
Conclusion: The findings from this study revealed that hexane fraction of C. afer leaves balm formula exhibited higher anti-inflammatory activity against xylene-induced topical inflammation.

Key words: Anti-inflammation; balm; cream; Costus afer; topical inflammation


 
ARTICLE TOOLS
Abstract
PDF Fulltext
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Godswill Nduka Anyasor
Articles by Olamidotun Henry Ijituyi
on Google
on Google Scholar
Article Statistics
 Viewed: 2998
Downloaded: 474


How to Cite this Article
Pubmed Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. A J Physiol Biochem Pharmacol. 2018; 7(2): 54-60. doi:10.5455/ajpbp.20180518112401


Web Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. http://www.ajpbp.com/?mno=299550 [Access: January 28, 2020]. doi:10.5455/ajpbp.20180518112401


AMA (American Medical Association) Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. A J Physiol Biochem Pharmacol. 2018; 7(2): 54-60. doi:10.5455/ajpbp.20180518112401



Vancouver/ICMJE Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. A J Physiol Biochem Pharmacol. (2018), [cited January 28, 2020]; 7(2): 54-60. doi:10.5455/ajpbp.20180518112401



Harvard Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi (2018) Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. A J Physiol Biochem Pharmacol, 7 (2), 54-60. doi:10.5455/ajpbp.20180518112401



Turabian Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. 2018. Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. American Journal of Physiology, Biochemistry and Pharmacology, 7 (2), 54-60. doi:10.5455/ajpbp.20180518112401



Chicago Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. "Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model." American Journal of Physiology, Biochemistry and Pharmacology 7 (2018), 54-60. doi:10.5455/ajpbp.20180518112401



MLA (The Modern Language Association) Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi. "Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model." American Journal of Physiology, Biochemistry and Pharmacology 7.2 (2018), 54-60. Print. doi:10.5455/ajpbp.20180518112401



APA (American Psychological Association) Style

Godswill Nduka Anyasor, Olamidotun Henry Ijituyi (2018) Formulated hexane fraction of Costus afer leaves balm suppressed xylene induced topical inflammation in rat model. American Journal of Physiology, Biochemistry and Pharmacology, 7 (2), 54-60. doi:10.5455/ajpbp.20180518112401





Most Viewed Articles
  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415

  • Selected disease fundamentals based on the unified cell bioenergetics
    Andrzej Kasperski, Renata Kasperska
    J Investig Biochem. 2013; 2(2): 93-100
    » Abstract & References » doi: 10.5455/jib.20130227041230

  • Prevalence of vitamin B12 deficiency in Helicobacter pylori infected patients in Jordan.
    Saad Abed Aljalil Alfawaeir, Mohammad Basim Abuzaid
    J Investig Biochem. 2013; 2(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20121215061954

  • Serum levels of high-sensitivity C-reactive protein (hs-CRP) in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Basim Abu Zaid
    J Investig Biochem. 2013; 2(1): 32-36
    » Abstract & References » doi: 10.5455/jib.20121225015606

  • Hepatoprotective and In vivo Anti-oxidant Activity of Costus afer Leaf Extracts against Acetaminophen-Induced Hepatotoxicity in Rats
    God'swill Nduka Anyasor, Kofoworola O. Odunsanya, Audrey C. Ibeneme
    J Investig Biochem. 2013; 2(1): 53-61
    » Abstract & References » doi: 10.5455/jib.20130301030851

  • Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children
    Saeed Thabet Nasher, Mohammed Wael Abu Ghoush
    J Investig Biochem. 2013; 2(1): 44-52
    » Abstract & References » doi: 10.5455/jib.20130105022014

  • Correlation Between Elevated Hepatic Enzymes & High-Sensitivity Crp in Metabolic Syndrome.
    Al-Rubaye Faisal, Shakir Mahmood, Jumah Kadhum
    J Investig Biochem. 2012; 1(1): 6-11
    » Abstract & References » doi: 10.5455/jib.20120528094732

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314

  • Alteration of antioxidant enzymes and oxidative stress in elderly patient.
    Seema L. Jawalekar1, Anil K. Bhutey2, Aarti Karnik3, Vasant T. Surve4
    J Investig Biochem. 2013; 2(1): 37-43
    » Abstract & References » doi: 10.5455/jib.20130129093838

  • Phytochemical content of Cnidoscolus aconitifolius leaves and toxicological effect of its aqueous leaf extract in Wistar rats
    Doris Akachukwu, Polycarp Nnacheta Okafor, Chiedozie O Ibegbulem
    J Investig Biochem. 2014; 3(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20140504023102

  • Most Downloaded
  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415

  • Preclinical Screening Techniques for Antidiarrheal Drugs: A comprehensive review
    Earnest Oghenesuvwe Erhirhie, Tedwins Joseph Oghenebrorie Emudainohwo, Sabastine Obiora Igboeme, Daniel Lottana Ajaghaku, Treasure Nonye Ujam, Ugochukwu Moses Okezie, Emmanuel Emeka Ilodigwe
    A J Physiol Biochem Pharmacol. 2018; 7(2): 61-74
    » Abstract » doi: 10.5455/ajpbp.20180329014330

  • Serum levels of high-sensitivity C-reactive protein (hs-CRP) in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Basim Abu Zaid
    J Investig Biochem. 2013; 2(1): 32-36
    » Abstract & References » doi: 10.5455/jib.20121225015606

  • Selected disease fundamentals based on the unified cell bioenergetics
    Andrzej Kasperski, Renata Kasperska
    J Investig Biochem. 2013; 2(2): 93-100
    » Abstract & References » doi: 10.5455/jib.20130227041230

  • Alteration of antioxidant enzymes and oxidative stress in elderly patient.
    Seema L. Jawalekar1, Anil K. Bhutey2, Aarti Karnik3, Vasant T. Surve4
    J Investig Biochem. 2013; 2(1): 37-43
    » Abstract & References » doi: 10.5455/jib.20130129093838

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314

  • Hepatoprotective and In vivo Anti-oxidant Activity of Costus afer Leaf Extracts against Acetaminophen-Induced Hepatotoxicity in Rats
    God'swill Nduka Anyasor, Kofoworola O. Odunsanya, Audrey C. Ibeneme
    J Investig Biochem. 2013; 2(1): 53-61
    » Abstract & References » doi: 10.5455/jib.20130301030851

  • Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children
    Saeed Thabet Nasher, Mohammed Wael Abu Ghoush
    J Investig Biochem. 2013; 2(1): 44-52
    » Abstract & References » doi: 10.5455/jib.20130105022014

  • Phytochemical content of Cnidoscolus aconitifolius leaves and toxicological effect of its aqueous leaf extract in Wistar rats
    Doris Akachukwu, Polycarp Nnacheta Okafor, Chiedozie O Ibegbulem
    J Investig Biochem. 2014; 3(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20140504023102

  • Comparative study of ischemia modified albumin and nitric oxide in hyperthyroidism
    Monica Verma1, Kiran Dahiya1, Veena Singh Ghalaut1, Abhishek Soni2, Jagjeet Singh3, Vasudha Dhupper1
    J Investig Biochem. 2014; 3(1): 17-20
    » Abstract & References » doi: 10.5455/jib.20130707090209

  • Most Cited Articles