E-ISSN 2578-7322
 

Original Research 


Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats

Victoria Chinenye Obinna, Hope Delesi Kagbo, Gabriel Ogaba Agu.

Abstract
Objectives: To supply scientific information / literature on the use of P.oleracea in enhancing fertility in males; either to validate or to refute this folkloric use. This study investigated the effect of the lipophilic and hydrophilic leaf extracts of Portulaca oleracea on male reproductive parameters.
Methods: The extracts were obtained using two extracting solvents – chloroform and 80% aqueous methanol in succession. Experimental animals were randomly divided into 7 groups of 16 rats each. Group A (control) received 0.5ml 20% Tween 80 (vehicle), groups B, C and D received 125, 250 and 500 mg/kg of the lipophilic extract respectively and E, F and G received 125, 250 and 500 mg/kg of the hydrophilic extract respectively for 60 days. On days 14, 28, 42 and 60; four rats from each group were weighed and anaesthetized for sample collection. Blood (for testosterone), testes (weight and histomorphology) and sperm cells from caudal epididymis (for semen analysis) were collected.
Results: Both extracts had no significant (p>0.05) effect on testicular weight and histomorphology, and sperm cell characteristics but caused a significant (p<0.05) increase in sperm count. Only the hydrophilic extract produced a significant (p<0.05) reduction in the testosterone level.
Conclusion: Leaf extracts of P.oleracea as used in this study improved spermatogenesis irrespective of the decline in testosterone level. This may justify the use of P.oleracea leaf extracts in enhancing fertility in males, although further studies are required to elucidate the mechanism and compounds associated with these properties.
extracts in enhancing fertility in males, although further studies are required to elucidate the mechanism and compounds associated with these properties.

Key words: Portulaca oleracea, sperm cell count, testosterone, testis, sperm morphology


 
ARTICLE TOOLS
Abstract
PDF Fulltext
Print this article Print this Article
How to cite this articleHow to cite this article
Citation Tools
Related Records
 Articles by Victoria Chinenye Obinna
Articles by Hope Delesi Kagbo
Articles by Gabriel Ogaba Agu
on Google
on Google Scholar
Article Statistics
 Viewed: 951
Downloaded: 112
Cited: 0


How to Cite this Article
Pubmed Style

Obinna VC, Kagbo HD, Agu GO, . Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. A J Physiol Biochem Pharmacol. 2019; 9(1): 21-32. doi:10.5455/ajpbp.20190124101830


Web Style

Obinna VC, Kagbo HD, Agu GO, . Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. http://www.ajpbp.com/?mno=27829 [Access: August 24, 2019]. doi:10.5455/ajpbp.20190124101830


AMA (American Medical Association) Style

Obinna VC, Kagbo HD, Agu GO, . Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. A J Physiol Biochem Pharmacol. 2019; 9(1): 21-32. doi:10.5455/ajpbp.20190124101830



Vancouver/ICMJE Style

Obinna VC, Kagbo HD, Agu GO, . Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. A J Physiol Biochem Pharmacol. (2019), [cited August 24, 2019]; 9(1): 21-32. doi:10.5455/ajpbp.20190124101830



Harvard Style

Obinna, V. C., Kagbo, . H. D., Agu, . G. O. & (2019) Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. A J Physiol Biochem Pharmacol, 9 (1), 21-32. doi:10.5455/ajpbp.20190124101830



Turabian Style

Obinna, Victoria Chinenye, Hope Delesi Kagbo, Gabriel Ogaba Agu, and . 2019. Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. American Journal of Physiology, Biochemistry and Pharmacology, 9 (1), 21-32. doi:10.5455/ajpbp.20190124101830



Chicago Style

Obinna, Victoria Chinenye, Hope Delesi Kagbo, Gabriel Ogaba Agu, and . "Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats." American Journal of Physiology, Biochemistry and Pharmacology 9 (2019), 21-32. doi:10.5455/ajpbp.20190124101830



MLA (The Modern Language Association) Style

Obinna, Victoria Chinenye, Hope Delesi Kagbo, Gabriel Ogaba Agu, and . "Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats." American Journal of Physiology, Biochemistry and Pharmacology 9.1 (2019), 21-32. Print. doi:10.5455/ajpbp.20190124101830



APA (American Psychological Association) Style

Obinna, V. C., Kagbo, . H. D., Agu, . G. O. & (2019) Effects of Lipophilic and Hydrophilic leaf extracts of Portulaca oleracea Linn. (Purslane) on male reproductive parameters in albino rats. American Journal of Physiology, Biochemistry and Pharmacology, 9 (1), 21-32. doi:10.5455/ajpbp.20190124101830





Most Viewed Articles
  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415

  • Selected disease fundamentals based on the unified cell bioenergetics
    Andrzej Kasperski, Renata Kasperska
    J Investig Biochem. 2013; 2(2): 93-100
    » Abstract & References » doi: 10.5455/jib.20130227041230

  • Prevalence of vitamin B12 deficiency in Helicobacter pylori infected patients in Jordan.
    Saad Abed Aljalil Alfawaeir, Mohammad Basim Abuzaid
    J Investig Biochem. 2013; 2(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20121215061954

  • Serum levels of high-sensitivity C-reactive protein (hs-CRP) in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Basim Abu Zaid
    J Investig Biochem. 2013; 2(1): 32-36
    » Abstract & References » doi: 10.5455/jib.20121225015606

  • Hepatoprotective and In vivo Anti-oxidant Activity of Costus afer Leaf Extracts against Acetaminophen-Induced Hepatotoxicity in Rats
    God'swill Nduka Anyasor, Kofoworola O. Odunsanya, Audrey C. Ibeneme
    J Investig Biochem. 2013; 2(1): 53-61
    » Abstract & References » doi: 10.5455/jib.20130301030851

  • Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children
    Saeed Thabet Nasher, Mohammed Wael Abu Ghoush
    J Investig Biochem. 2013; 2(1): 44-52
    » Abstract & References » doi: 10.5455/jib.20130105022014

  • Correlation Between Elevated Hepatic Enzymes & High-Sensitivity Crp in Metabolic Syndrome.
    Al-Rubaye Faisal, Shakir Mahmood, Jumah Kadhum
    J Investig Biochem. 2012; 1(1): 6-11
    » Abstract & References » doi: 10.5455/jib.20120528094732

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314

  • Alteration of antioxidant enzymes and oxidative stress in elderly patient.
    Seema L. Jawalekar1, Anil K. Bhutey2, Aarti Karnik3, Vasant T. Surve4
    J Investig Biochem. 2013; 2(1): 37-43
    » Abstract & References » doi: 10.5455/jib.20130129093838

  • Potential Role of Oxidized LDL (ox-LDL) and Adhesion Molecules (VCAM-1, ICAM-1) in Type 2 Diabetes Mellitus Patients in Qassim region, KSA
    Manal Basyouni, Maha Ahmed, Hisham Ismail , Iman Esmat
    J Investig Biochem. 2012; 1(1): 48-54
    » Abstract & References » doi: 10.5455/jib.20120417052458

  • Most Downloaded
  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415

  • Preclinical Screening Techniques for Antidiarrheal Drugs: A comprehensive review
    Earnest Oghenesuvwe Erhirhie, Tedwins Joseph Oghenebrorie Emudainohwo, Sabastine Obiora Igboeme, Daniel Lottana Ajaghaku, Treasure Nonye Ujam, Ugochukwu Moses Okezie, Emmanuel Emeka Ilodigwe
    A J Physiol Biochem Pharmacol. 2018; 7(2): 61-74
    » Abstract » doi: 10.5455/ajpbp.20180329014330

  • Serum levels of high-sensitivity C-reactive protein (hs-CRP) in Helicobacter pylori infected patients
    Saad Al-Fawaeir, Mohammad Basim Abu Zaid
    J Investig Biochem. 2013; 2(1): 32-36
    » Abstract & References » doi: 10.5455/jib.20121225015606

  • Selected disease fundamentals based on the unified cell bioenergetics
    Andrzej Kasperski, Renata Kasperska
    J Investig Biochem. 2013; 2(2): 93-100
    » Abstract & References » doi: 10.5455/jib.20130227041230

  • Alteration of antioxidant enzymes and oxidative stress in elderly patient.
    Seema L. Jawalekar1, Anil K. Bhutey2, Aarti Karnik3, Vasant T. Surve4
    J Investig Biochem. 2013; 2(1): 37-43
    » Abstract & References » doi: 10.5455/jib.20130129093838

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314

  • Hepatoprotective and In vivo Anti-oxidant Activity of Costus afer Leaf Extracts against Acetaminophen-Induced Hepatotoxicity in Rats
    God'swill Nduka Anyasor, Kofoworola O. Odunsanya, Audrey C. Ibeneme
    J Investig Biochem. 2013; 2(1): 53-61
    » Abstract & References » doi: 10.5455/jib.20130301030851

  • Comparative study of ischemia modified albumin and nitric oxide in hyperthyroidism
    Monica Verma1, Kiran Dahiya1, Veena Singh Ghalaut1, Abhishek Soni2, Jagjeet Singh3, Vasudha Dhupper1
    J Investig Biochem. 2014; 3(1): 17-20
    » Abstract & References » doi: 10.5455/jib.20130707090209

  • Evaluation of glycated albumin and microalbuminuria as early risk markers of nephropathy in type 2 diabetes mellitus
    Suresh Babu Kondaveeti, Ivvala Anand Shaker
    J Investig Biochem. 2013; 2(2): 127-131
    » Abstract & References » doi: 10.5455/jib.20130507061739

  • Molecular Characterization of Alpha and Beta Thalassemia in Yemeni Sickle Cell Disease Children
    Saeed Thabet Nasher, Mohammed Wael Abu Ghoush
    J Investig Biochem. 2013; 2(1): 44-52
    » Abstract & References » doi: 10.5455/jib.20130105022014

  • Most Cited Articles
  • Biochemical study on the impact of Nigella sativa and virgin olive oils on cadmium-induced nephrotoxicity and neurotoxicity in rats
    Eman Taha Mohammed, Khalid Shaban Hashem, Mahmoud Ramadan Abdel Rheim
    J Investig Biochem. 2014; 3(2): 71-78
    » Abstract & References » doi: 10.5455/jib.20140716041908
    Cited : 8 times [Click to see citing articles]

  • Alterations in plasma lipids, glutathione and homocysteine in relation to dietary copper in rats
    Magda A. Megahed1, Kamel M. A. Hassanin2, Ibrahim M. I. Youssef3, Abdul Baset A. Elfghi4, Kamal A. Amin2
    J Investig Biochem. 2014; 3(1): 21-25
    » Abstract & References » doi: 10.5455/jib.20130716075753
    Cited : 6 times [Click to see citing articles]

  • Study and Characterization of the Antioxidant Activity of the Inclusion Complex of Apigenin with β-Cyclodextrin and HP-β-Cyclodextrin in solution
    Jinxia Li
    J Investig Biochem. 2014; 3(3): 107-111
    » Abstract & References » doi: 10.5455/jib.20140905032504
    Cited : 5 times [Click to see citing articles]

  • Phytochemical content of Cnidoscolus aconitifolius leaves and toxicological effect of its aqueous leaf extract in Wistar rats
    Doris Akachukwu, Polycarp Nnacheta Okafor, Chiedozie O Ibegbulem
    J Investig Biochem. 2014; 3(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20140504023102
    Cited : 5 times [Click to see citing articles]

  • Slippery Elm, its Biochemistry, and use as a Complementary and Alternative Treatment for Laryngeal Irritation
    Christopher R Watts, Bernard rousseau
    J Investig Biochem. 2012; 1(1): 17-23
    » Abstract & References » doi: 10.5455/jib.20120417052415
    Cited : 4 times [Click to see citing articles]

  • Serum Lipid Profile of Breast Cancer Patients in Kashmir
    Showkat Ahmad Bhat, Manzoor R Mir, Sabhiya Majid, Ahmad Arif Reshi, Ishraq Husain, Tehseen Hassan, Hilal Ahmad
    J Investig Biochem. 2013; 2(1): 26-31
    » Abstract & References » doi: 10.5455/jib.20121125075314
    Cited : 4 times [Click to see citing articles]

  • Quercetin, a natural phytochemical and antioxidant protects against sodium azide-induced hepatic and splenic oxidative stress in rats
    Oluwatobi Temitope Somade, Oluseyi Adeboye Akinloye, Michael Oluwayemi Adeyeye, Gbenga Daniel Fabunmi, Olakunle Opeoluwa Idowu, Funmilayo Oluwaseun Badmus, Barakat Oluwatoyin Salaudeen
    J Investig Biochem. 2015; 4(3): 69-74
    » Abstract & References » doi: 10.5455/jib.20151220014400
    Cited : 3 times [Click to see citing articles]

  • A New Marker For The Diagnosýs Of Sepsis: Presepsin
    Mehmet Agilli, Irfan Sener, Fatih Yesildal, Tevfik Honca, Ibrahim Aydin, Emin Ozgur Akgul, Halil Yaman
    J Investig Biochem. 2012; 1(1): 55-57
    » Abstract & References » doi: 10.5455/jib.20120521073837
    Cited : 3 times [Click to see citing articles]

  • The role of urinary 5- hydroxyindoleacetic acid determination in diagnosis of acute appendicitis
    Hamid Hindi Sarhan, Aseel Fawzi Hatroosh, Amina Hamed Alobaidi
    J Investig Biochem. 2013; 2(1): 1-5
    » Abstract & References » doi: 10.5455/jib.20121026084836
    Cited : 3 times [Click to see citing articles]

  • Myeloperoxidase, Malondialdehyde and serum Lipids in type 2 diabetes mellitus
    Mohamedi Begum, Ashok Kumar J., Hilda Priya D'Souza, Sushith Sushith, Prathima M.B, Reshma S., Shashikala M.D, Manjula Anil, Suriyan S Nair, Kavitha Ashok Kumar
    J Investig Biochem. 2015; 4(1): 13-17
    » Abstract & References » doi: 10.5455/jib.20150331115356
    Cited : 3 times [Click to see citing articles]