Study And Ch Aracterization Of The Antioxidant Activity Of The Inclusion Complex Of Apigenin With Cyclodextrin And 2Hydr

Abstract

Jinxia Li, Kenm ing Yu, Jianping Bai, Huizhi Zhang, Jianbin Chao

Aim: To determine the interaction of AP with ß-cyclodextrin (ß-CD) and 2-hydroxylpropyl (HP)-ß-CD was studied in detail based on fluorescence method and to determined the effect of the complex process on their antioxidant capacity. Methods: The formation of the complexes of apigenin (AP) with ß-cyclo dextrin (ß-CD) and 2-hydroxylpropyl (HP)-ß-CD was studied by fluorescence spectra in solution. The formation constants (Ks) of complexes were determined by fluorescence method and phase-solubility measurements. Results: The results showed that the inclusion ability of ß-CD and its derivative were the order: HP-ß-CD>ß-CD. In addition, the experimental resulted confirmed the existence of 1:1 inclusion complex of AP with CDs. Kinetic st udies of scavenging of 1, 1-Diphenyl2-picryl-hydrazyl free radical with AP and CDs complexes were done. Conclusion: The resul ts obtained indicated that the AP/HP-ß-CD complex was the most reactive form, then was ß-CD complex, and last was free AP.