Potential role of PAX5 in Breast cancer: Relation to CD19

Abstract

Manal Basyouni Ahmed, Muneera Al-Sheeha , Maha Imam Ahmed, Enas Samir Nabih

Background: PAX5 is a transcriptional factor which is considered as one of the key molecules for regulation of many cancer associated genes. Objectives: To examine the status of PAX5 in human breast carcinoma and evaluate its role via CD19. Patients and Methods: PAX5 and CD19 expression in breast tissue samples were measured by RT-PCR in 90 female breast specimens including 50 cases of invasive ductal carcinoma, 25 cases with fibroadenoma and 15 non-pathological breast tissue as control group. Results: The best cut-off values for the investigated markers were determined by ROC curve. PAX5 at 0.05 had 92.0% sensitivity and 92.5% specificity while CD19 at 0.62 had 94.0% sensitivity and 95.0% specificity. Moreover, mean levels and positivity rates for PAX5 and CD19 showed significant difference among the three investigated groups (p< 0.001). PAX5 was higher in advanced grades (p< 0.05), stages (p< 0.01) and negative estrogen tumor receptors (p< 0.05). Additionally, PAX5 showed a significant positive correlation with CD19. Conclusion: PAX5 may be a potential biomarker having a possible role in breast cancer tumorgenesis through CD19.